Methylation, which contributesto epigenetic inheritance, can occur either through DNA methylation or protein methylation. DNA methylation in vertebrates typically occurs at CpG sites, which results in the conversion of the cytosine to 5-methylcytosine. 1-2% of the human genome has CpG clusters and there is an inverse relationship between CpG methylation and transcriptional activity. Therefore, quantitative methylation analyses have become an effective approach for cancer gene studies.
CpG island can be detected in DNA using a variety of assay techniques, including Southern blot analysis (SB), RE-PCR, bisulfite genomic sequencing (BGS), bisulfite modification followed by the polymerase chain reaction, and pyrosequencing.
Applied Gene provides an innovative polymerase chain reaction based platform, which combines real-time PCR and high resolution melting (HRM) analyses for both global and gene-specific DNA methylation quantification.

Applied Gene’s CpG Methylation services offer these benefits:

Preliminary CpG screening on various species and sample types;
Cost-effective, high-throughput quantitative methylation analyses;
Availability of custom assay

Sample
A customer submits bisulfite modified DNA ( click here for details on sample requirement/shipments.)

Applied Gene Delivers
Applied Gene performs real-time PCR and High Resolution Melting analyses

Result

Results available in an electronic form or on a CD.

Please contact technical support for details or click Here for sample requirement.