Real-Time polymerase chain reaction (PCR) is one of the most powerful and sensitive gene analysis techniques available and is used for a broad range of applications including quantitative gene expression analysis, genotyping, SNP analysis, pathogen detection, drug target validation and for measuring RNA interference. Frequently, real-time PCR is combined with reverse transcription to quantify messenger RNA (mRNA) and MicroRNA (miRNA) in cells or tissues. Real-time PCR based gene expression study has been widely used on determining the difference in the relative quantity of a transcript expressed in different samples, quantifying gene expression levels of cells or tissues, and determining copy numbers.

The cost of a gene expression project can vary from a couple of hundred dollars to tens of thousand dollars, depending on the sample amount and the number of genes of interest. To support the gene expression profiling applications, Applied Gene offers medium throughput gene expression services with affordable prices. Applied Gene provides GLP-compliant and PCR- based gene expression with flexible gene/sample combinations. Contact Applied Gene Experts to discuss your project and get the price quote.

Gene Expression Services Benefits:

1. Flexible and medium throughput gene expression with affordable prices;
2. Rapid turnaround time within 3~5 business days;
3. Availability of custom assays;
4. Reliable real-time PCR available for:

• TaqMan® chemistry, which uses a fluorogenic probe to enable the detection of a specific PCR product as it accumulates during PCR cycles.
• SYBR® Green I dye chemistry, which uses SYBR® Green I dye—a highly specific, double-stranded DNA binding dye—to detect PCR product as it accumulates during PCR cycles

5. GLP-compliant services.

Service Types:

Standard: A complete PCR plate send over to AG, AG runs instrument and do standard data analysis.

Option1: Primers, reagent and cDNA are sent to AG separately, AG prepares PCR plate, runs instrument, and do data analysis

Option2: Only cDNA is sent over, AG provides primers, reagnents, and prepare PCR plate. AG runs instrument, and do data analysis.

Please contact technical support for details or click Here for sample requirement.